COVID19 Convalescent plasma

We present a labeled large-scale, high resolution chest x-ray dataset for the automated exploration of medical images along with their associated reports. This dataset includes more than 160,000 images obtained from 67,000 patients that were interpreted and reported by radiologists at San Juan Hospital (Spain) from 2009 to 2017, covering six different position views and additional information on image acquisition and patient demography. The reports were labeled with 174 different radiographic findings, 19 differential diagnoses and 104 anatomic locations organized as a hierarchical taxonomy and mapped onto standard Unified Medical Language System (UMLS) terminology. Of these reports, 27% were manually annotated by trained physicians and the remaining set was labeled using a supervised method based on a recurrent neural network with attention mechanisms. The labels generated were then validated in an independent test set achieving a 0.93 Micro-F1 score. To the best of our knowledge, this is one of the largest public chest x-ray databases suitable for training supervised models concerning radiographs, and the first to contain radiographic reports in Spanish. The PadChest dataset can be downloaded from http//bimcv.cipf.es/bimcv-projects/padchest/.
To investigate the latent factors underlying signs of childhood apraxia of speech (CAS) in a group of 57 children with CAS.
The speech of 57 children with CAS (aged 3;5-17;0) was coded for signs of CAS. All participants showed at least five signs of CAS and were judged to have CAS by speech pathologists experienced in pediatric speech disorders. Participants were selected to represent a range of severity of CAS 30 children were verbal and 27 were minimally verbal with comorbid autism. Participants' scores for each sign (the number of times that sign appeared during a child's speech sample) were converted to z-scores, then entered as variables into an exploratory factor analysis. Models were compared using the Akaike Information Criterion (AIC).
The three-factor model had the lowest AIC and best fit the data. After oblique rotation, syllable segmentation, slow rate, and stress errors loaded most highly on Factor 1. Groping, addition of phonemes other than schwa, and difficulty with coarticulation loaded most highly on Factor 2. Variable errors loaded most highly on Factor 3. Thus, factors were interpreted as being associated with (1) prosody, (2) coarticulation, and (3) inconsistency.
Results are consistent with the three consensus criteria for CAS from the American Speech-Language-Hearing Association Inappropriate prosody, disrupted coarticulatory transitions, and inconsistent errors on repeated tokens. High loading of the syllable segmentation sign on the inappropriate prosody factor also supports the use of a pause-related biomarker for CAS.
Results are consistent with the three consensus criteria for CAS from the American Speech-Language-Hearing Association Inappropriate prosody, disrupted coarticulatory transitions, and inconsistent errors on repeated tokens. High loading of the syllable segmentation sign on the inappropriate prosody factor also supports the use of a pause-related biomarker for CAS.Parasites of Leishmania genus have developed various strategies to overcome host immune response favoring its infection and development toward leishmaniasis. With an array of virulence factors, those parasites modify host macrophage signaling and functions. Depending of the species involved, visceral or cutaneous leishmaniasis will develop. Several years ago, Leishmania Viannia guyanensis that is naturally infected with the endosymbiotic virus Leishmania RNA Virus 1 was found to cause a particularly aggressive form of South-American mucocutaneous leishmaniasis. This virus, when co-transmitted with the parasite was shown to strongly modulate RNA sensors and NLRP3 inflammasome network that could explain in part the exacerbated skin pathology caused by this particular parasite. TG100115 In this review, we will be discussing how this endosymbiotic virus-infected Leishmania in conjunction with Leishmania exosomes partner together to manipulate host immune response in their favor.This study aimed to reveal the impact of energy restriction on the intestine via structural and molecular changes in terms of intestinal stem cell (ISC) function, ISC niche, intestinal epithelial barrier function, and intestinal immune function. Female C57BL/6J mice, aged 12 months, fed a commercial chow were used in this study. The ISC function, ISC niche, intestinal epithelial barrier function, and intestinal immune function were assessed. Energy restriction reversed aging-induced intestinal shortening and made the crypts shallower. The intestinal epithelial cells isolated from the intestine showed a significant increase in the expression levels of stem cell-associated genes in small intestinal epithelial cells as detected by flow cytometry. Despite the increase in the number of stem cells and the expression levels of markers, no increase or decrease was found in the enteroid complexity of the small intestine and colonic enteroid formation in vitro. The colonic mucous layer was measured in mice of the energy restricted (ER)-treated group to investigate the epithelial barrier function in the colon. The results revealed that the barrier was more complete. The fluorescence intensity of tight junction markers claudin-2 and zonula occludens-1 increased and the mRNA expression profiles of monocyte chemotactic protein 1 and interleukin-6 decreased in the colon of mice in the ER-treated group. The beneficial effects of ER on the colon in terms of the integrity of the mucosal barrier and alleviation of inflammation were confirmed, thus highlighting the importance of modulating the intestinal function in developing effective antiaging dietary interventions.Within the domains of both science and religion, beliefs in unobservable phenomena - such as bacteria or the soul - are common. Yet given the radically different trajectory of scientific as compared to religious beliefs across human history, it is plausible that the psychological basis for beliefs in these two domains is also different. Indeed, there is evidence from children and adults in various cultures that people have greater confidence in their scientific beliefs than in their religious beliefs. However, when individuals are invited to indicate the basis for their beliefs within each domain, a surprisingly similar pattern of justification is apparent.Many religions emphasize the importance of sexual morality. This article argues mating strategies are central to understanding religion. I highlight the reproductive-religiosity model, which suggests that religious behavior is partly motivated by preferences for restricted mating strategies. I then discuss how religion can lead to reproductive benefits. Specifically, religions can make parenting a relatively safer strategy by increasing paternal certainty, which drives men toward parental investment, and alloparenting, which reduces offspring mortality rates. Next, I discuss the social implications of reproductive-religiosity, including mate selection and trust. Finally, I discuss the potential role of mating strategies in the evolution and cultural evolution of religion and discuss future directions for developing an approach to religion rooted in mating interests.The visualization of glycogen deposits in cells and tissues is important for studying glycogen metabolism as well as diagnosis of glycogen storage diseases. Evidence suggests that the demonstration of glycogen can better be enhanced by factors such the choice of fixative and temperature during fixation. Here, we assessed efficacy of neutral buffered formalin (NBF), alcoholic formalin (AF) and paraformaldehyde (PFA) at 4 °C, 37 °C and 40 °C using Periodic Acid Schiff's staining method. Each liver specimen was fixed in NBF and AF while the brain tissues were fixed in NBF, AF and PFA. We found that there was a better PAS staining intensity with the liver tissues fixed in AF compared with NBF. Also, there was no difference in the quality of the staining for tissues fixed in AF at 37 °C, 4 °C and 40 °C, but fixation with NBF at 4 °C gave the best staining quality when compared with 40 °C and 37 °C. Furthermore, hippocampal tissues fixed in AF showed better quality of PAS staining compared with NBF and PFA. A significant increase in staining intensity was observed for PFA when compared with NBF. Superior staining intensity for PAS was observed at 4 °C for hippocampal tissues fixed with NBF, AF and PFA. Taken together our results show that AF at a temperature of 4 °C gave the best result. Hence, glycogen demonstration can better be enhanced by the choice of fixative and temperature during fixation.Cerebral palsy (CP) is a serious disorder that has serious consequences on the functioning of children and their families. This study explores family functioning (adaptation, cohesion and family type) and strengths (pride, accord and whole) in Greek and Italian families raising a child with CP and their interaction. Participants were 120 parents of a biological child with CP. Data collection used a self-report questionnaire, the Family Adaptability and Cohesion Evaluation Scale (FACES-III) and the Family Strengths Inventory. According to the findings, parents (in both countries) estimate and wish their family to be in the balanced range which represents healthy family functioning according to the revised edition of the Olson Circumplex Model. Finally, they share a high sense of family strengths, which is mainly displayed in the high sense of pride and accord.This study investigated the feasibility of polysaccharide-coated poly(n-butyl cyanoacrylate) (PBCA) nanoparticles for oral delivery of acyclovir (ACV). PBCA nanoparticles were obtained by the emulsion polymerization method. Chitosan was chemically modified to obtain N,N,N-trimethylchitosan (TMC), which was used to coat the nanoparticles (PBCA-TMC). Nanoparticles were characterized by dynamic light scattering, zeta potential, differential scanning calorimetry (DSC), atomic force microscopy (AFM), cytotoxicity, and the effect on the transepithelial electrical resistance (TEER) of the Caco-2 cells. The size of the coated nanoparticles (296.2 nm) was significantly larger than uncoated (175.0 nm). Furthermore, PBCA nanoparticles had a negative charge (-11.7 mV), which was inverted to highly positive values (+36.5 mV) after coating. DSC analysis suggested the occurrence of the coating, which was confirmed by AFM images. The MTT assay revealed concentration-dependent cytotoxicity for the core-shell nanoparticles. Additionally, PBCA-TMC caused a significant but reversible decrease in the Caco-2 cell monolayer TEER. Entrapped ACV (PBCA-ACV-TMC), a Biopharmaceutical Classification System class III drug substance, increased approximately 3.25 times the Papp of ACV in the Caco-2 permeability assay. The nanoparticles were also able to provide in vitro ACV controlled release using media with different pH values (1.2; 6.8; 7.4). Accordingly, this new core-shell nanoparticle showed the potential to improve the oral delivery of ACV.Electrochemical biointerfaces are constructed with a wide range of nanomaterials and conducting polymers that strongly affect the analytical performance of biosensors. The analysis of progress toward electrochemical sensing platforms offers opportunities to provide devices for commercial use. The investigation of different methods for the synthesis of phenol biointerfaces leads to design challenges in the field of monitoring phenolic compounds. This paper review the innovative strategies and feature techniques in the construction of phenolic compound biosensors. The focus was made on the preparation methods of nanostructures and nanomaterials design for catalytic improvements of sensing interfaces. The paper also provides a comprehensive overview in the field of enzyme immobilization approaches at solid supports and technical formation of polymer nanocomposites, as well as applications of hybrid organic-inorganic nanocomposites in phenolic biosensors. This review also highlights the recent progress in the electrochemical detection of phenolic compounds and summarizes analytical performance parameters including sensitivity, storage stability, limit of detection, linear range, and Michaelis-Menten kinetic analysis.