Concepts and issues throughout heart vasomotor function screening

This identified five proteins (mTurquoise2, mNeonGreen, mClover3, mKOκ, and tdTomato) that are robustly expressed and detectable in mesophyll cells of stably transformed plants. Using microparticle bombardment, we show that mTurquoise2 and mNeonGreen can be used for simultaneous multicolor imaging of different subcellular compartments. Overall, we conclude that mTurquoise2, mNeonGreen, mClover3, mKOκ, and tdTomato are suitable for high-resolution live imaging of rice leaves, both after transient and stable transformation. Along with the rapid microparticle bombardment method, which allows transient transformation of major cell types in the leaf blade, these fluorescent reporters should greatly facilitate the analysis of gene expression and cell biology in rice. © 2020 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.Background Diets higher in protein have been reported to improve age-related changes in body composition via increased energy expenditure, shifts in substrate oxidation (SO), and decreased appetite. However, how protein source (e.g., animal compared with plant protein) affects energy expenditure, appetite, and food intake as we age is unknown. Objectives The objective of this study was to evaluate the effect of protein source as part of a high-protein breakfast on appetite, food intake, energy expenditure, and fat oxidation in young men (YM) compared with older men (OM). Methods This study used a randomized, single-blinded crossover design, with a 1-wk washout period between testing days. Fifteen YM (mean ± SD age 25.2 ± 2.8 y) and 15 OM (67.7 ± 4.5 y), healthy adults, participated in the study. Participants arrived fasted and consumed an isocaloric, volume-matched, high-protein (40-g) test beverage made with either an animal [whey protein isolate (WPI)] or plant [pea protein isolate (PPI)] protein isolate source. Markers of appetite and energy expenditure were determined at baseline and over 4 h postprandial. Results There was a significant effect of time, age, and protein source on appetite (P  less then  0.05). There was no effect of protein source on plasma markers of appetite, food intake, energy expenditure, and SO. After controlling for body weight, OM had decreased energy expenditure (P  less then  0.05) and lower fat oxidation (P  less then  0.001) compared with YM. Conclusions This study indicates that a high-protein breakfast containing WPI or PPI exerts comparable effects on appetite, energy expenditure, and 24-h energy intake in both young and older healthy adult men.This trial was registered at clinicaltrials.gov as NCT03399812. Copyright © The Author(s) 2020.Background There is a variety of specialized nutritious foods available for use in programs targeting undernutrition, but evidence supporting the choice of product is limited. Objectives We compared the cost-effectiveness of 4 specialized nutritious foods to prevent stunting and wasting in children aged 6-23 mo in Burkina Faso. Methods Four geographic regions were randomly assigned to 1 of 4 intervention arms Corn-Soy Blend Plus (CSB+) programmed with separate fortified vegetable oil (the reference food), Corn-Soy-Whey Blend (CSWB; a new formulation) with oil, SuperCereal Plus (SC+), and ready-to-use supplementary food (RUSF). We compared the effects of each intervention arm on growth (length-for-age z score (LAZ), weight-for-length z score (WLZ), end-line stunting (LAZ less then -2), and total monthly measurements of wasting (WLZ less then -2). Rations were ∼500 kcal/d, distributed monthly. selleck compound Children were enrolled in the blanket supplementary feeding program at age ∼6 mo and measured monthly for ∼18 mo. A) 2020.Traumatic intrasubstance ruptures of the biceps brachii are rare. Injury to its tendinous insertion or origin is most common. Isolated short head injuries are rare, and musculocutaneous nerve involvement has been reported for these injuries. We present a unique case report of a young, healthy man who sustained a symptomatic median and musculocutaneous nerve compression resulting from an intrasubstance tear of the short head of the biceps after a snow blower injury. Short belly rupture with injury-associated scar tissue compression of both the median and musculocutaneous nerves was identified in the operating room. Microsurgical decompression and tendon repair with a modified Mason-Allen configuration using 0-Vicryl suture were done. By 11 months postoperative, the patient experienced resolution of his arm pain, extension and flexion improvement from 0 to +140 to +15 to +150, elimination of the Tinel sign and of concomitant arm deformity, and improvement of elbow strength. Copyright © 2019 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Orthopaedic Surgeons.The purpose of this study was to report and compare early outcomes during the first 3 months of the recovery phase in patients who underwent primary total hip arthroplasty (THA) with direct anterior approach (DAA) and posterior approach (PA). Background The DAA for primary THA has gained popularity within the past few years. Although controversy exists regarding the long-term benefit when compared with the PA, several authors have reported markedly better outcomes in the early recovery weeks, when using DAA. Methods For this study, data were prospectively collected and retrospectively reviewed for all primary THAs from March 2014 to October 2017. Included patients underwent primary THA through DAA or PA and had minimum 3-month postoperative measures for the Harris Hip Score, Forgotten Joint Score-12, Veterans RAND 12 Mental (VR-12 Mental), Veterans RAND 12 Physical (VR-12 Physical), 12-Item Short-Form (SF) Survey Mental, 12-Item SF Survey Physical (SF-12 Physical), Visual Analog Scale, and patient satisfaction. An analysis using propensity score matching was done to establish the DAA and PA groups. Matching (11 ratio) was conducted based on the following covariates age, sex, body mass index, and laterality. Results Twenty-four DAA THA patients were successfully matched using propensity scoring to 24 PA THA patients. The DAA group demonstrated significantly higher scores for the following patient-reported outcome scores Harris Hip Score, VR-12 Mental, VR-12 Physical, and SF-12 Physical (P = 0.0090, P = 0.0388, P = 0.0063, and P = 0.0132, respectively). Conclusion At 3-month follow-up, both the DAA and PA groups reported favorable outcomes after THA. However, the DAA group scored markedly higher regarding quality-of-life outcomes when compared with a propensity score-matched group of PA patients. Copyright © 2019 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Orthopaedic Surgeons.