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In this mass analyzer, pulsed ions are introduced into a separation space during a pause period of the alternating electric field. Furthermore, as defined, the measured ionic species exits from the separation space during the next pause period after receiving the action of the alternating electric field for one period. At this time, the ions of the measured ionic species are energy-focused and separated from the others by the difference in displacement caused by the electric field. In the concept section, the mass analyzer and the alternating electric field were illustrated, and the conditions that the measured ionic species should satisfy were clarified. An equation giving the displacement magnitude of the measured ionic species after one period was derived, showing that the mass separation is performed based on m/z. An equation giving the mass resolution was derived from this equation. In the simulation and discussion section, the mass separation in the alternating electric field and the energy focusing of the ions of the measured ionic species were demonstrated by numerical calculations. A scanning method that changes the period of the alternating electric field was demonstrated, showing that there is no limit to the m/z range that can be analyzed. Besides, it was demonstrated that ionic species in a required m/z range can be analyzed simultaneously with the introduction of one packet of pulsed ions. It was shown that measurement operations can be repeated with no or a little wasted time. Complementarily, the direction-focusing performance of the electric sector was examined.A zero-background method based on surface-enhanced Raman scattering (SERS) was developed for the rapid determination of cymoxanil residue in food. Because of the influence of complex matrices, conventional Raman spectroscopy has multiple peaks that overlap with those of target molecules, which makes qualitative and quantitative detection difficult. However, the cyano group (C≡N) of cymoxanil after ultraviolet irradiation has a special characteristic peak in the Raman-silent region (1800-2800 cm-1), which eliminates the possible background interference. The intensity of the characteristic peak at 2130 cm-1 exhibited a good linear relationship (R2 = 0.9907) with the concentration of cymoxanil in the range of 1.0-50.0 mg/L, whose limit of detection was 0.5 mg/L. The novel method was also applied to the detection of cymoxanil residue in real samples such as cucumber and grape, and the results were in good agreement with those from high-performance liquid chromatography analysis. This revealed that the SERS method has great potential in the detection of cymoxanil in fruits and vegetables. Moreover, ultraperformance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-QTOF/MS) was adopted to identify the photoproducts of cymoxanil. The photolysis mechanism was explored by SERS and the UPLC-QTOF/MS technique, which provided basic information on photodegradation of cymoxanil.The aim of this study was to investigate six food-grade peptidase preparations, namely, Flavourzyme 1000L, Protease P "Amano" 6SD, DeltazymAPS-M-FG, Promod278, ProteAX-K, and Peptidase R, regarding their use for the hydrolysis of soy, pea, and canola protein. The relationship between the specific peptidase activities and, first, the degree of hydrolysis, second, the free amino acid profiles of the hydrolysates, and, third, the corresponding taste of the hydrolysates was analyzed using a random forest model. The taste attributes bitter and umami were of special interest. The peptidase ProteAX-K was the biocatalyst most suited for the high umami and low bitter taste of the plant-based protein hydrolysates based on the experimental results and the random forest model.Cell-cell communication plays a vital role in biological activities; in particular, membrane-protein interactions are profoundly significant. In order to explore the underlying mechanism of intercellular signaling pathways, a full range of artificial systems have been explored. However, many of them are complicated and uncontrollable. Ruboxistaurin clinical trial Herein we designed an artificial signal transduction system able to control the influx of environmental ions by triggering the activation of synthetic transmembrane channels immobilized on giant membrane vesicles (GMVs). A membrane protein-like stimulator from one GMV community (GMVB) stimulates a receptor on another GMV community (GMVA) to release ssDNA messengers, resulting in the activation of synthetic transmembrane channels to enable the influx of ions. This event, in turn, triggers signal responses encapsulated in the GMVA protocell model. By mimicking natural signal transduction pathways, this novel prototype provides a workable tool for investigating cell-cell communication and expands biological signaling systems in general as well as explores useful platforms for addressing scientific problems which involve materials science, chemistry, and medicine.Organosilanes are synthetically useful reagents and precursors in organic chemistry. However, the typical inertness of unactivated Si-C(sp3) bonds under conventional reaction conditions has hampered the application of simple tetraalkylsilanes in organic synthesis. Herein we report the chemoselective cleavage of Si-C(sp3) bonds of unactivated tetraalkylsilanes using iodine tris(trifluoroacetate). The reaction proceeds smoothly under mild conditions (-50 °C to room temperature) and tolerates various polar functional groups, thus enabling subsequent Tamao-Fleming oxidation to provide the corresponding alcohols. NMR experiments and density functional theory calculations on the reaction indicate that the transfer of alkyl groups from Si to the I(III) center and the formation of the Si-O bond proceed concertedly to afford an alkyl-λ3-iodane and silyl trifluoroacetate. The developed method enables the use of unactivated tetraalkylsilanes as highly stable synthetic precursors.Peptides are regarded as promising next-generation therapeutics. However, an analysis of over 1000 bioactive peptide candidates suggests that many have underdeveloped affinities and could benefit from cyclization using a bridging linker sequence. Until now, the primary focus has been on the use of inert peptide linkers. Here, we show that affinity can be significantly improved by enriching the linker with functional amino acids. We engineered a peptide inhibitor of PCSK9, a target for clinical management of hypercholesterolemia, to demonstrate this concept. Cyclization linker optimization from library screening produced a cyclic peptide with ∼100-fold improved activity over the parent peptide and efficiently restored low-density lipoprotein (LDL) receptor levels and cleared extracellular LDL. The linker forms favorable interactions with PCSK9 as evidenced by thermodynamics, structure-activity relationship (SAR), NMR, and molecular dynamics (MD) studies. This PCSK9 inhibitor is one of many peptides that could benefit from bioactive cyclization, a strategy that is amenable to broad application in pharmaceutical design.