Postoperative hallux varus overview of treatment procedures

In spite of this scale amendments, only three patients could be treated differently because of the change in the stage of the disease.
Changing the treatment method, including withdrawal from surgery, can help avoid unnecessary treatment, but on the other hand, may potentially reduce the patient's chances of survival by depriving them of the possibility of radical treatment.
Changing the treatment method, including withdrawal from surgery, can help avoid unnecessary treatment, but on the other hand, may potentially reduce the patient's chances of survival by depriving them of the possibility of radical treatment.
Non-small cell lung cancer (NSCLC) accounts for more than 80% of lung cancer. CircRNA is a new type of non-coding RNA. CircRNA was found to be deeply involved in the regulation of NSCLC cells. However, the principle of CircRNA regulating NSCLC cells needs to be further explored.
The relative mRNA expression levels of CircRNA_103993, miR-1271 and ERG were detected by quantificational real-time polymerase chain reaction (qRT-PCR) in NSCLC cells and human bronchial epithelial cell line HBE. Cell proliferation was detected by the Cell Counting Kit (CCK8) when NSCLC cells were transfected with si-CircRNA_103993, si-NC, miR-1271 mimics, miR-NC, LV-ERG, respectively. The apoptotic rate of NSCLC cells was measured by apoptotic assay and flow Cytometry. The relative mRNA and protein expression levels of PCNA and caspase-3 were detected by Western blot and qRT-PCR.
CircRNA_103993 and ERG were significantly up-regulated while miR-1271 was significantly down-regulated in NSCLC cells. Knockdown of CircRNA_103993 andR-1271. The CircRNA_103993 /miR-1271/ ERG axis had an important effect on the proliferation and apoptosis of NSCLC cells. Therefore, CircRNA_103993 may be a target for treating lung cancer.
CircRNA_103993 was highly expressed in NSCLC cells. CircRNA_103993 regulated proliferation and apoptosis of NSCLC cells by acting as a sponge of miR-1271. The CircRNA_103993 /miR-1271/ ERG axis had an important effect on the proliferation and apoptosis of NSCLC cells. Therefore, CircRNA_103993 may be a target for treating lung cancer.
To study the expression and biological functions of long intergenic non-protein coding ribonucleic acid 00355 (LINC00355) in gastric cancer (GC), and to explore its potential mechanism of action.
The relative expression level of LINC00355 in 48 cases of GC tissues, the corresponding paracancerous tissues, and GC cells was determined using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and the interference efficiency of small interfering (si)-LINC00355 was detected via qRT-PCR. After knock-down of LINC00355, methyl thiazolyl tetrazolium (MTT) and 5-ethynyl-2'-deoxyuridine (EdU) assays were performed to detect the changes in the proliferation ability of GC cells, and the changes in the GC cell cycle distribution and apoptosis rate were examined by flow cytometry. Besides, Western blotting was conducted to verify the changes in the downstream signaling pathway of LINC00355.
Among 48 cases of GC tissues, there were 42 (87.5%) cases of LINC00355 expression up-regulation, and 6 (12.5%) cases of LINC00355 expression down-regulation. The qRT-PCR results revealed that the expression of LINC00355 was raised in 4 kinds of GC cells. After interference with LINC00355 expression, the MTT assay results indicated that the cell proliferation ability was inhibited, consistent with the EdU assay results. dWIZ-2 After LINC00355 was knocked down in GC cells, GC cells in experiment group had a higher apoptosis rate than those in si-NC group and arrested in the gap 0 (G0)/G1 phase. Moreover, it was found through Western blotting that the expressions of the molecular markers in the downstream wingless-INT (Wnt)/β-catenin signaling pathway were downregulated after interference with the expression of LINC00355.
LINC0035 exhibits an up-regulated expression in GC and regulates the Wnt/β-catenin signaling pathway to promote proliferation and inhibit apoptosis.
LINC0035 exhibits an up-regulated expression in GC and regulates the Wnt/β-catenin signaling pathway to promote proliferation and inhibit apoptosis.
Cholangiocarcinoma (CCA) is one of the tumors with high malignancy of the liver and bile system, whose development and prognosis mechanisms are still not clear. Here, a preliminary illustration was made on the expression and function of long non-coding RNA (lncRNA) CASC2 and the relevant mechanism of its function.
Expression of CASC2 in CCA tissues and cells were examined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Cell proliferation ability was detected using colony formation and Cell Counting Kit-8 (CCK-8) assays while cell invasion and migration abilities were measured using transwell and Matrigel assays. Using bioinformatic analysis, underlying downstream molecules of CASC2 were predicted and by Dual-Luciferase assay and Western blot.
It was found that CASC2 was expressed at a significantly lower level in CCA tissues and cell lines. The overexpression of CASC2 inhibited QBC939 cell proliferation, invasion and migration when the knockdown of CASC2 accelerated HUCCT1 cell growth and metastasis. Besides, miR-18a was identified as a direct target for CASC2, and SOCS5 as target for miR-18a. Moreover, CASC2 functioned as a sponge of miR-18a to promote the SOCS5 expression, then, slowed down the epithelial-to-mesenchymal transition (EMT) progression.
CASC2 was downregulated in CCA tissues and cells. It could inhibit cell proliferation, invasion, migration and EMT via sponging miR-18a/SOCS5 axis. This might provide a novel target for CCA diagnosis and treatment.
CASC2 was downregulated in CCA tissues and cells. It could inhibit cell proliferation, invasion, migration and EMT via sponging miR-18a/SOCS5 axis. This might provide a novel target for CCA diagnosis and treatment.
Primary gallbladder carcinoma (GBC) is one of the most common biliary malignancies in the gastrointestinal tract. In this work, we examined the roles of circular-mitochondrial translation optimization 1 (circ-MTO1) in GBC tissues and patient plasma.
Circ-MTO1 expression in GBC tissues and patient plasma was evaluated by quantitative Real Time-PCR (qRT-PCR). The relationships between circ-MTO1 expression and the pathological characteristics of GBC were analyzed. Kaplan-Meier survival curve was applied to calculate overall survival (OS) and progression-free survival (PFS) in GBC patients with different circ-MTO1 expression. The univariate COX regression curve analysis method was employed to analyze the potential relationships between high circ-MTO1 expression and OS and PFS. At last, we assessed the diagnostic value of the circ-MTO1 level in GBC patient plasma.
Circ-MTO1 expression was significantly upregulated in tumor tissues and plasma in GBC patients. In addition, circ-MTO1 expression was associated with clinical-pathological characteristics in GBC.